Testicles should be of the same size and of firm not hard consistency. Normal Semen should have the following characteristics:
There are 4 hormones that is responsible for ovulation:
Estrogen cannot be used to indicate ovulation. Progesterone is used for that purpose, levels of 2 to 3 ng corresponds with LH surge which leads to ovulation 48 hours later. The ova take another 48 hours to mature after being released. Only then they are ready for fertilization. Ovulation corresponds with Progesterone levels of 5 ng. The due date is 63 days from the day of ovulation and has nothing to do with the breeding date.
Below is a video of Lihuen receiving surgical insemination to achieve pregnancy.